Role of Microbial Diversity in Textile Dye Degradation: A Comprehensive Review

Authors

  • Hadia Islam Author
  • Jaweria Ashraf Author
  • Umm-e-Umayma Author
  • Yusra Noor Author
  • Areesha Mansoor Author
  • Huba Yasin Author
  • Jaweria Marium Author
  • Ayisha Aman Ullah Jinnah University for Women image/svg+xml Author

DOI:

https://doi.org/10.66173/jenmas.2026.55

Keywords:

Dye Degradation, Biodegradation, Textile Dyes, Microbial Degradation, Wastewater Treatment, Industrial Effluents

Abstract

About 80% of the dyes from the textile industry are directly dumped into the water bodies without prior treatment. This leads to the accumulation of these harmful compounds in the water bodies, which causes serious damage to the biota. The current physicochemical processes for effluent treatment have limitations due to high energy consumption, inaccurate technical optimization, and chemical cost. Moreover, the by-products of these processes are usually more harmful than the original dyes. Biotechnology offers a promising solution to this issue through the application of microbes for dye degradation. This approach allows cost-effective, eco-friendly detoxification without the excessive production of toxic by-products. Enzymatic degradation and biosorption are the two major strategies by which microbial-based dye degradation is carried out. This review describes the challenges posed by synthetic dyes and how various microbial species (bacteria, algae, fungi, and yeast) are involved in their degradation. Various emerging technologies, including microbial consortia, engineered microbes, material-assisted, and artificial intelligence (AI) based degradation strategies, are being developed for enhanced microbial efficiency. This review will demonstrate these cutting-edge dye degradation techniques, important bioreactor configurations used for this purpose, and the future directions towards the implementation of sustainable detoxification processes. 

References

[1] Alegbe, E. O., & Uthman, T. O. (2024). A review of history, properties, classification, applications and challenges of natural and synthetic dyes. Heliyon, 10(13). https://doi.org/10.1016/j.heliyon.2024.e33646

[2] Lin, J., Ye, W., Xie, M., Seo, D. H., Luo, J., Wan, Y., & Van der Bruggen, B. (2023). Environmental impacts and remediation of dye-containing wastewater. Nature Reviews Earth & Environment, 4(11), 785-803. https://doi.org/10.1038/s43017-023-00489-8

[3] Lellis, B., Fávaro-Polonio, C. Z., Pamphile, J. A., & Polonio, J. C. (2019). Effects of textile dyes on health and the environment and bioremediation potential of living organisms. Biotechnology research and innovation, 3(2), 275-290. https://doi.org/10.1016/j.biori.2019.09.001

[4] Emanuele, L., & D’Auria, M. (2024). The use of heterocyclic azo dyes on different textile materials: a review. Organics, 5(3), 277-289. https://doi.org/10.3390/org5030015

[5] Alzain, H., Kalimugogo, V., Hussein, K., & Karkadan, M. (2023). A review of environmental impact of azo dyes. Int J Res Rev, 10(6), 673-689. https://doi.org/10.52403/ijrr.20230682

[6] Tomar, T., Kahandawala, N., Kaur, J., Thounaojam, L., Choudhary, I., & Bera, S. (2023). Bioremediation of synthetic dyes from wastewater by using microbial nanocomposites: An emerging field for water pollution management. Biocatalysis and Agricultural Biotechnology, 51, 102767. https://doi.org/10.1016/j.bcab.2023.102767

[7] Periyasamy, A. P. (2025). A review of bioremediation of textile dye containing wastewater. Cleaner Water, 4, 100092. https://doi.org/10.1016/j.clwat.2025.100092

[8] Hossen, A., Chowdhury, T., & Mondal, I. (2022). Purification of textile dye-contained wastewater by three alternative promising techniques: Adsorption, Biodegradation and Advanced Oxidation Processes (AOPs)-A review. J Textile Eng Fashion Technol, 8(3), 96-98. https://doi.org/10.15406/jteft.2022.08.00306

[9] Castellanos‐Henriquez, C. S., Rosso, D., Chávez‐Parga, M. D. C., & Cortés, J. A. (2026). A Review of Advanced Oxidation Processes Applied to the Removal of Reactive Dyes: Efficiency, Removal Conditions, Advantages, Disadvantages, and Industrial Effluents Applications. Water Environment Research, 98(2), e70283. https://doi.org/10.1002/wer.70283

[10] Kuppusamy, B., Mohamed Ismail, F. R., Balakrishnan, P., Kim, S. C., Asrafali, S. P., & Periyasamy, T. (2026). From Biomass to Adsorbent: A Comprehensive Review on Bio-Derived Carbons for Dye Removal. Polymers, 18(2), 180. https://doi.org/10.3390/polym18020180

[11] Najim, A. A., Radeef, A. Y., & Jabbar, Z. H. (2025). Recent trends in physio-chemo technologies and their role in dyes removal: Effectiveness, benefits, and limitations. Chemical Engineering Research and Design, 219, 198-221. https://doi.org/10.1016/j.cherd.2025.06.005

[12] Ismail, G. A., & Sakai, H. (2024). Toxicity changes of dye degradation via photo-fenton treatment and the possible degradation mechanism. Case Studies in Chemical and Environmental Engineering, 9, 100665. https://doi.org/10.1016/j.cscee.2024.100665

[13] Pandey, A., Kumar, S., Bithel, N., Kumar, S., & Mir, M. A. (2025). Sustainable biodegradation of malachite green dye by novel non-pathogenic Pseudomonas aeruginosa ED24. World Journal of Microbiology and Biotechnology, 41(2), 44. https://doi.org/10.1007/s11274-025-04251-8

[14] Haque, M. M., Hossen, M. N., Rahman, A., Roy, J., Talukder, M. R., Ahmed, M., ... & Haque, M. A. (2024). Decolorization, degradation and detoxification of mutagenic dye Methyl orange by novel biofilm producing plant growth-promoting rhizobacteria. Chemosphere, 346, 140568. https://doi.org/10.1016/j.chemosphere.2023.140568

[15] El-Bendary, M. A., Fawzy, M. E., Abdelraof, M., El-Sedik, M., & Allam, M. A. (2023). Efficient malachite green biodegradation by Pseudomonas plecoglossicide MG2: process optimization, application in bioreactors, and degradation pathway. Microbial Cell Factories, 22(1), 192. https://doi.org/10.1186/s12934-023-02194-z

[16] Tahir, M., Usman, M., Naqqash, T., Shahid, M., Farooq, A. B. U., Hassan, M. M., ... & Khan, U. (2025). Brilliant green and Congo red degradation efficiency of the azoreductase gene-inhabiting Klebsiella aerogenes and Enterobacter sp. strains. International Journal of Biological Macromolecules, 148289. https://doi.org/10.1016/j.ijbiomac.2025.148289

[17] Tripathi, M., Singh, P., Singh, R., Bala, S., Pathak, N., Singh, S., ... & Singh, P. K. (2023). Microbial biosorbent for remediation of dyes and heavy metals pollution: a green strategy for sustainable environment. Frontiers in Microbiology, 14, 1168954. https://doi.org/10.3389/fmicb.2023.1168954

[18] Afrin, S., Shuvo, H. R., Sultana, B., Islam, F., Rus'd, A. A., Begum, S., & Hossain, M. N. (2021). The degradation of textile industry dyes using the effective bacterial consortium. Heliyon, 7(10). https://doi.org/10.1016/j.heliyon.2021.e08102

[19] Lade, H., Kadam, A., Paul, D., & Govindwar, S. (2015). Biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes. EXCLI journal, 14, 158. https://doi.org/10.1515/aep-2016-0042

[20] Ali, A., Abid, H. M. U., Karim, K., Ruzieva, M., Arsalan, Z., Arshad, M. A., ... & Saidmuratova, M. (2025). Unlocking the Power of Enzymes: Comparative Molecular Docking Analysis of Escherichia. Coli Enzymes for Disperse Dye Degradation and Toxicity Mitigation. Desalination and Water Treatment, 101429. https://doi.org/10.1016/j.dwt.2025.101429

[21] Oyewusi, H. A., Wahab, R. A., Akinyede, K. A., Albadrani, G. M., Al-Ghadi, M. Q., Abdel-Daim, M. M., ... & Huyop, F. (2024). Bioinformatics analysis and molecular dynamics simulations of azoreductases (AzrBmH2) from Bacillus megaterium H2 for the decolorization of commercial dyes. Environmental Sciences Europe, 36(1), 31. https://doi.org/10.21203/rs.3.rs-3615118/v1

[22] Mabuza, L., Sonnenberg, N., & Marx-Pienaar, N. (2023). Natural versus synthetic dyes: Consumers' understanding of apparel coloration and their willingness to adopt sustainable alternatives. Resources, Conservation & Recycling Advances, 18, 200146. https://doi.org/10.1016/j.rcradv.2023.200146

[23] Samsami, S., Mohamadizaniani, M., Sarrafzadeh, M. H., Rene, E. R., & Firoozbahr, M. (2020). Recent advances in the treatment of dye-containing wastewater from textile industries: Overview and perspectives. Process safety and environmental protection, 143, 138-163. https://doi.org/10.1016/j.psep.2020.05.034

[24] Mishra, S., & Maiti, A. (2018). The efficacy of bacterial species to decolourise reactive azo, anthroquinone and triphenylmethane dyes from wastewater: a review. Environmental Science and Pollution Research, 25(9), 8286-8314. https://doi.org/10.1007/s11356-018-1273-2

[25] Routoula, E., & Patwardhan, S. V. (2020). Degradation of anthraquinone dyes from effluents: a review focusing on enzymatic dye degradation with industrial potential. Environmental science & technology, 54(2), 647-664. https://doi.org/10.1021/acs.est.9b03737

[26] Harish, B. S., Thayumanavan, T., Nambukrishnan, V., & Sakthishobana, K. (2023). Heterogeneous biocatalytic system for effective decolorization of textile dye effluent. 3 Biotech, 13(6), 165. https://doi.org/10.1007/s13205-023-03586-z

[27] Batool, S., Akib, S., Ahmad, M., Balkhair, K. S., & Ashraf, M. A. (2014). Study of modern nano enhanced techniques for removal of dyes and metals. Journal of Nanomaterials, 2014(1), 864914. https://doi.org/10.1155/2014/864914

[28] Ayub, A., Wani, A. K., Chopra, C., Sharma, D. K., Amin, O., Wani, A. W., ... & Singh, R. (2025). Advancing dye degradation: integrating microbial metabolism, photocatalysis, and nanotechnology for eco-friendly solutions. Bacteria, 4(1), 15. https://doi.org/10.3390/bacteria4010015

[29] Khan, A., Nayarisseri, A., & Singh, S. K. (2025). Characterization and optimization of azo dyes degrading microbes isolated from textile effluent. Scientific Reports, 15(1), 11241. https://doi.org/10.1038/s41598-025-95359-2

[30] Mishra, A., Takkar, S., Joshi, N. C., Shukla, S., Shukla, K., Singh, A., ... & Varma, A. (2022). An integrative approach to study bacterial enzymatic degradation of toxic dyes. Frontiers in Microbiology, 12, 802544. https://doi.org/10.3389/fmicb.2021.802544

[31] Moyo, S., Makhanya, B. P., & Zwane, P. E. (2022). Use of bacterial isolates in the treatment of textile dye wastewater: A review. Heliyon, 8(6). https://doi.org/10.1016/j.heliyon.2022.e09632

[32] Ouagued, I., Cretin, M., Petit, E., Lesage, G., Djafer, A., Ouagued, A., & Lacour, S. (2025). Screening refractory dye degradation by different advanced oxidation processes. Molecules, 30(3), 712. https://doi.org/10.3390/molecules30030712

[33] Espina, G., Cáceres-Moreno, P., Mejías-Navarrete, G., Ji, M., Sun, J., & Blamey, J. M. (2021). A novel and highly active recombinant spore-coat bacterial laccase, able to rapidly biodecolorize azo, triarylmethane and anthraquinonic dyestuffs. International journal of biological macromolecules, 170, 298-306. https://doi.org/10.1016/j.ijbiomac.2020.12.123

[34] Du, L., Wu, H., Li, G., Wei, Y., Wang, F., Xu, L., & Dong, X. (2023). Efficient degradation and decolorization of triphenylmethane dyes by Serratia sp. WKD under extreme environmental conditions and the mechanism. International Biodeterioration & Biodegradation, 179, 105565. https://doi.org/10.1016/j.ibiod.2023.105565

[35] Tyagi, S., Singh, R., Kapoor, R. T., Jain, A., & Shah, M. P. (2026). Efficient decolorization and phytotoxicity reduction of textile azo dye acid red 114 by an in-situ isolated bacterium Paenibacillus dendritiformis: a sustainable approach. Archives of Microbiology, 208(1), 69. https://doi.org/10.1007/s00203-025-04604-9

[36] Elnabi, M. K. A., Ghazy, M. A., Ali, S. S., Eltarahony, M., & Nassrallah, A. (2025). Efficient biodegradation and detoxification of reactive black 5 using a newly constructed bacterial consortium. Microbial Cell Factories, 24(1), 154. https://doi.org/10.1186/s12934-025-02768-z

[37] Purnomo, A. S., Hairunnisa, F. W., Maria, V. P., Rohmah, A. A., Putra, S. R., Putro, H. S., & Rizqi, H. D. (2024). Anionic dye removal by immobilized bacteria into alginate-polyvinyl alcohol-bentonite matrix. Heliyon, 10(6). https://doi.org/10.1016/j.heliyon.2024.e27871

[38] Rane, A., & Joshi, S. J. (2021). Biodecolorization and biodegradation of dyes: A review. The Open Biotechnology Journal, 15(1). https://doi.org/10.2174/1874070702115010097

[39] John, J., Dineshram, R., Hemalatha, K. R., Dhassiah, M. P., Gopal, D., & Kumar, A. (2020). Bio-decolorization of synthetic dyes by a halophilic bacterium Salinivibrio sp. Frontiers in Microbiology, 11, 594011. https://doi.org/10.3389/fmicb.2020.594011

[40] Qiu, H., Shen, F., Yin, A., Liu, J., Wu, B., Li, Y., ... & Xu, B. (2022). Biodegradation and detoxification of azo dyes by halophilic/halotolerant microflora isolated from the salt fields of Tibet autonomous region China. Frontiers in Microbiology, 13, 877151. https://doi.org/10.3389/fmicb.2022.877151

[41] Zammuto, V., Macrì, A., Agostino, E., Ruggeri, L. M., Caccamo, M. T., Magazù, S., ... & Gugliandolo, C. (2024). Enhancement of biodegradation and detoxification of methylene blue by preformed biofilm of thermophilic bacilli on polypropylene perforated balls. Journal of Marine Science and Engineering, 12(8), 1248. https://doi.org/10.3390/jmse12081248

[42] Tian, F., Guo, G., Liu, C., Hao, J., Ding, K., Yang, F., ... & Abolfathi, S. (2025). Biodegradation of azo dyes by a newly enriched extreme-thermophilic bacterial consortium. Journal of Water Process Engineering, 79, 108839. https://doi.org/10.1016/j.jwpe.2025.108839

[43] Pavithran, K., Ramasamy, P., & Jagadeesan, M. (2025). Harnessing Coastal Halophiles for Azo Dye Biodegradation: Phylogenetic and Spectroscopic Evidence of Efficient Bioremediation. Journal of Environmental Chemical Engineering, 118150. https://doi.org/10.1016/j.jece.2025.118150

[44] Eshghi, S., & Jookar Kashi, F. (2025). Acid Red 88 biodegradation by Cu nanoparticles stabilized on Marinospirillum alkaliphilum strain N. Scientific Reports, 15(1), 18903. https://doi.org/10.1038/s41598-025-03427-4

[45] Prasad, A. A., Satyanarayana, V. S. V., & Rao, K. B. (2013). Biotransformation of Direct Blue 1 by a moderately halophilic bacterium Marinobacter sp. strain HBRA and toxicity assessment of degraded metabolites. Journal of hazardous materials, 262, 674-684. https://doi.org/10.1016/j.jhazmat.2013.09.011

[46] Touliabah, H. E. S., El-Sheekh, M. M., Ismail, M. M., & El-Kassas, H. (2022). A review of microalgae-and cyanobacteria-based biodegradation of organic pollutants. Molecules, 27(3), 1141. https://doi.org/10.3390/molecules27031141

[47] Kohlheb, N., van Afferden, M., Lara, E., Arbib, Z., Conthe, M., Poitzsch, C., ... & Becker, M. Y. (2020). Assessing the life-cycle sustainability of algae and bacteria-based wastewater treatment systems: High-rate algae pond and sequencing batch reactor. Journal of environmental management, 264, 110459. https://doi.org/10.1016/j.jenvman.2020.110459

[48] González, V., Abalde, J., & Torres, E. (2024). Discoloration and biosorption of Brilliant green dye in seawater using living biomass of the microalga Phaeodactylum tricornutum. Journal of Applied Phycology, 36(4), 1823-1835. https://doi.org/10.1007/s10811-024-03200-6

[49] Sigamani, S., Chinnasamy, R., Sathiyamoorthy, T., Narayanasamy, M., Nagarajan, S., Ramamurthy, D., & Natarajan, H. (2024). Eco-friendly biodegradation of synthetic dyes using algae and its toxicological assessment on Clarias gariepinus. Biomass Conversion and Biorefinery, 14(16), 19835-19848. https://doi.org/10.1007/s13399-023-04208-7

[50] Patel, H., Yadav, V. K., Yadav, K. K., Choudhary, N., Kalasariya, H., Alam, M. M., ... & Jeon, B. H. (2022). A Recent and Systemic Approach towards Microbial Biodegradation of Dyes from Textile Industries. Water 2022, 14, 3163. https://doi.org/10.3390/w14193163

[51] Nair, V. K., Selvaraju, K., Samuchiwal, S., Naaz, F., Malik, A., & Ghosh, P. (2023). Phycoremediation of synthetic dyes laden textile wastewater and recovery of bio-based pigments from residual biomass: an approach towards sustainable wastewater management. Processes, 11(6), 1793. https://doi.org/10.3390/pr11061793

[52] Oruganti, R. K., Katam, K., Show, P. L., Gadhamshetty, V., Upadhyayula, V. K. K., & Bhattacharyya, D. (2022). A comprehensive review on the use of algal-bacterial systems for wastewater treatment with emphasis on nutrient and micropollutant removal. Bioengineered, 13(4), 10412-10453. https://doi.org/10.1080/21655979.2022.2056823

[53] Anand, U., Dey, S., Parial, D., Federici, S., Ducoli, S., Bolan, N. S., ... & Bontempi, E. (2023). Algae and bacteria consortia for wastewater decontamination and transformation into biodiesel, bioethanol, biohydrogen, biofertilizers and animal feed: a review. Environmental Chemistry Letters, 21(3), 1585-1609. https://doi.org/10.1007/s10311-023-01562-w

[54] Duong, D. V., Trung, L. D., Tuong, L. Q., My, L. V. H., Tuyet, L. T. H., & Tran, T. (2024, December). Green treatment solutions: Decolorization of textile wastewater by Chlorella vulgaris. In IOP Conference Series: Earth and Environmental Science (Vol. 1419, No. 1, p. 012020). IOP Publishing. https://doi.org/10.1088/1755-1315/1419/1/012020

[55] Majhi, P. K., Kothari, R., Pandey, A., & Tyagi, V. V. (2021). Adsorptive behavior of free and immobilized Chlorella pyrenoidosa for decolorization. Biomass Conversion and Biorefinery, 11(6), 3023-3036. https://doi.org/10.1007/s13399-020-00770-6

[56] Shaikh, W. A., Chakraborty, S., Islam, R. U., Ghfar, A. A., Naushad, M., Bundschuh, J., ... & Mondal, N. K. (2022). Fabrication of biochar-based hybrid Ag nanocomposite from algal biomass waste for toxic dye-laden wastewater treatment. Chemosphere, 289, 133243. https://doi.org/10.1016/j.chemosphere.2021.133243

[57] Abdelfattah, A., Ali, S. S., Ramadan, H., El-Aswar, E. I., Eltawab, R., Ho, S. H., ... & Sun, J. (2023). Microalgae-based wastewater treatment: Mechanisms, challenges, recent advances, and future prospects. Environmental science and ecotechnology, 13, 100205.https://doi.org/10.1016/j.ese.2022.100205

[58] Bilal, M., Rasheed, T., Sosa-Hernández, J. E., Raza, A., Nabeel, F., & Iqbal, H. M. (2018). Biosorption: an interplay between marine algae and potentially toxic elements—a review. Marine drugs, 16(2), 65. https://doi.org/10.3390/md16020065

[59] Shetty, K., & Krishnakumar, G. (2020). Algal and cyanobacterial biomass as potential dye biodecolorizing material: a review. Biotechnology letters, 42(12), 2467-2488. https://doi.org/10.1007/s10529-020-03005-w

[60] Góralczyk-Bińkowska, A., Długoński, A., Bernat, P., Długoński, J., & Jasińska, A. (2021). Environmental and molecular approach to dye industry waste degradation by the ascomycete fungus Nectriella pironii. Scientific Reports, 11(1), 23829. https://doi.org/10.1038/s41598-021-03446-x

[61] Mathur, M., Gola, D., Panja, R., Malik, A., & Ahammad, S. Z. (2018). Performance evaluation of two Aspergillus spp. for the decolourization of reactive dyes by bioaccumulation and biosorption. Environmental Science and Pollution Research, 25(1), 345-352. https://doi.org/10.1007/s11356-017-0417-0

[62] Anahid, S., Yaghmaei, S., & Ghobadinejad, Z. (2011). Heavy metal tolerance of fungi. Scientia Iranica, 18(3), 502-508. https://doi.org/10.1016/j.scient.2011.05.015

[63] Gostinčar, C., Stajich, J. E., & Gunde-Cimerman, N. (2023). Extremophilic and extremotolerant fungi. Current Biology, 33(14), R752-R756. https://doi.org/10.1016/j.cub.2023.06.011

[64] Chaurasia, P. K., Bharati, S. L., Sharma, N., Kumar, J., & Sivalingam, A. M. (2025). Degradation of dyes by fungi: An overview on recent updates. The Microbe, 6, 100232. https://doi.org/10.1016/j.microb.2024.100232

[65] Upadhyay, R., Przystaś, W., & Dave, B. (2025). Myco-remediation of synthetic dyes: a comprehensive review on contaminant alleviation mechanism, kinetic study and toxicity analysis. International Journal of Environmental Science and Technology, 22(1), 521-538. https://doi.org/10.1007/s13762-024-05793-4

[66] Mohammed, N. N., Zamel, D., Etman, A. E., Rabee, M. M., Elmasry, S. A., & Khan, A. U. (2024). Elucidation of the biodegradation mechanisms of fungi in efficient pollutant removal from wastewater. Studies in Fungi, 9(1). https://doi.org/10.48130/sif-0024-0011

[67] Gül, Ü. D., & Silah, H. (2014). Comparison of color removal from reactive dye contaminated water by systems containing fungal biosorbent, active carbon and their mixture. Water science and technology, 70(7), 1168-1174. https://doi.org/10.2166/wst.2014.339

[68] Costa, F. C., Brito, F. S., Grandi, M. J. R. D., & Daniel, J. F. (2025). White Rot Fungi for Biodegradation of Dyes: Potential for Industrial Uses-A Review. Journal of the Brazilian Chemical Society, 36(6), e-20250024. https://doi.org/10.21577/0103-5053.20250024

[69] Kiran, S., Huma, T., Jalal, F., Farooq, T., Hameed, A., Gulzar, T., ... & Rafique, M. A. (2019). Lignin degrading system of Phanerochaete chrysosporium and its exploitation for degradation of synthetic dyes wastewater. Pol. J. Environ. Stud, 28(3), 1749-1757. https://doi.org/10.15244/pjoes/89575

[70] Fayyaz, I., Saddick, S., Mahmood, R. T., Asad, M. J., Hussain, M. A., Hu, J., ... & Saydaxmetova, S. (2025). Biodegradation of Azo and disperse dyes by Trametes versicolor: Process optimization and MnP enzyme dynamics. Results in Engineering, 25, 103980. https://doi.org/10.1016/j.rineng.2025.103980

[71] Negi, B. B., Znad, H., & Das, C. (2025). Dual-functional waste fungal pellets of Phanerochaete chrysosporium for textile wastewater treatment: A waste to treat waste strategy. Sustainable Materials and Technologies, e01616. https://doi.org/10.1016/j.susmat.2025.e01616

[72] Asgher, M., Jamil, F., & Iqbal, H. M. N. (2012). Bioremediation potential of mixed white rot culture of Pleurotus ostreatus IBL-02 and Coriolus versicolor IBL-04 for textile industry wastewater. Journal of Bioremediation and Biodegradation S, 1, 007. https://doi.org/10.4172/2155-6199.s1-007

[73] Torres-Duarte, C., & Vazquez-Duhalt, R. (2010). Applications and prospective of peroxidase biocatalysis in the environmental field. In Biocatalysis based on heme peroxidases: peroxidases as potential industrial biocatalysts (pp. 179-206). Berlin, Heidelberg: Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-12627-7_8

[74] Al-Tohamy, R., Kenawy, E. R., Sun, J., & Ali, S. S. (2020). Performance of a newly isolated salt-tolerant yeast strain Sterigmatomyces halophilus SSA-1575 for azo dye decolorization and detoxification. Frontiers in Microbiology, 11, 1163. https://doi.org/10.3389/fmicb.2020.01163

[75] Kumar, M., Mishra, A., Patel, S. K., Kushwaha, J., Singh, S., Mishra, V., ... & Singh, D. (2025). Environmental Impacts and Strategies for Bioremediation of Dye-Containing Wastewater. Bioengineering, 12(10), 1043. https://doi.org/10.3390/bioengineering12101043

[76] Ngo, A. C. R., & Tischler, D. (2022). Microbial degradation of azo dyes: approaches and prospects for a hazard-free conversion by microorganisms. International journal of environmental research and public health, 19(8), 4740. https://doi.org/10.3390/ijerph19084740

[77] Carrascal-Hernández, D. C., Orozco-Beltrán, E. J., Insuasty, D., Márquez, E., & Grande-Tovar, C. D. (2025). Systematic evaluation of biodegradation of azo dyes by microorganisms: efficient species, physicochemical factors, and enzymatic systems. International Journal of Molecular Sciences, 26(16), 7973. https://doi.org/10.3390/ijms26167973

[78] Ali, M. M., Nassar, S., Nafady, N. A., & Mohamed, E. M. (2025). Bioremediation of malachite green dye toxicity under optimized conditions by Rhodotorula mucilaginosa AUMC13567. BMC biotechnology, 25(1), 39. https://doi.org/10.1186/s12896-025-00977-3

[79] Martorell, M. M., Pajot, H. F., & de Figueroa, L. I. (2017). Biological degradation of Reactive Black 5 dye by yeast Trichosporon akiyoshidainum. Journal of Environmental Chemical Engineering, 5(6), 5987-5993. https://doi.org/10.1016/j.jece.2017.11.012

[80] Dantroliya, S., Doshi, P., Raval, I., Joshi, C., & Joshi, M. (2024). Integrating azo dye degradation and lipid accumulation by Candida tropicalis and Pichia kudriavzevii along with insights into underlying metabolomics for treatment of textile effluents. Biochemical Engineering Journal, 212, 109521. https://doi.org/10.1016/j.bej.2024.109521

[81] Aziz, R., Saad, M., Tahir, H., & Aziz, R. (2024). Synthesis of NFO-immobilized yeast nanobiocomposite for ultrasound-assisted photo-fenton degradation of methylene blue by using central composite design. Surfaces and Interfaces, 44, 103725. https://doi.org/10.1016/j.surfin.2023.103725

[82] Tsilo, P. H., Basson, A. K., Ntombela, Z. G., Maliehe, T. S., & Pullabhotla, V. R. (2022). Production and characterization of a bioflocculant from Pichia kudriavzevii MH545928. 1 and Its Application in Wastewater Treatment. International journal of environmental research and public health, 19(6), 3148. https://doi.org/10.3390/ijerph19063148

[83] Cao, Z., Yan, W., Ding, M., & Yuan, Y. (2022). Construction of microbial consortia for microbial degradation of complex compounds. Frontiers in Bioengineering and Biotechnology, 10, 1051233. https://doi.org/10.3389/fbioe.2022.1051233

[84] Mohanty, S. S., & Kumar, A. (2025). Microbial decolorization of anthraquinone dyes: batch and continuous treatment systems-a mini-review. Frontiers in Environmental Engineering, 4, 1553712 https://doi.org/10.3389/fenve.2025.1553712

[85] Vieira, G. A. L., Cabral, L., Otero, I. V. R., Ferro, M., De Faria, A. U., de Oliveira, V. M., ... & Sette, L. D. (2021). Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis. Chemosphere, 267, 129190. https://doi.org/10.1016/j.chemosphere.2020.129190

[86] Tizazu, S., Tesfaye, G., Wang, A., Guadie, A., & Andualem, B. (2023). Microbial diversity, transformation and toxicity of azo dye biodegradation using thermo-alkaliphilic microbial consortia. Heliyon, 9(6). https://doi.org/10.1016/j.heliyon.2023.e16857

[87] Nanjani, S., Paul, D., & Keharia, H. (2021). Genome analysis to decipher syntrophy in the bacterial consortium 'SCP’for azo dye degradation. BMC microbiology, 21(1), 177. https://doi.org/10.1186/s12866-021-02236-9

[88] Aragaw, T. A., Suarez, C., Simachew, A., & Paul, C. J. (2025). The potential of alkaline tolerant microbial consortia for textile wastewater treatment under integrated anaerobic/aerobic conditions: Performance evaluation and microbial community analysis. International Biodeterioration & Biodegradation, 196, 105939. https://doi.org/10.1016/j.ibiod.2024.105939

[89] Sharma, M., Agarwal, S., Agarwal Malik, R., Kumar, G., Pal, D. B., Mandal, M., ... & Gupta, V. K. (2023). Recent advances in microbial engineering approaches for wastewater treatment: a review. Bioengineered, 14(1), 2184518. https://doi.org/10.1080/21655979.2023.2184518

[90] Khandare, R. V., Rane, N. R., Waghmode, T. R., & Govindwar, S. P. (2012). Bacterial assisted phytoremediation for enhanced degradation of highly sulfonated diazo reactive dye. Environmental Science and Pollution Research, 19(5), 1709-1718. https://doi.org/10.1007/s11356-011-0679-x

[91] Gayathiri, E., Prakash, P., Selvam, K., Awasthi, M. K., Gobinath, R., Karri, R. R., ... & Ravindran, B. (2022). Plant microbe based remediation approaches in dye removal: a review. Bioengineered, 13(3), 7798-7828. https://doi.org/10.1080/21655979.2022.2049100

[92] Zeb, B. S., Mahmood, Q., Irshad, M., Zafar, H., & Wang, R. (2025). Sustainable treatment of combined industrial wastewater: Synergistic phytoremediation with Eichhornia crassipes, Pistia stratiotes, and Arundo donax in biofilm wetlands. International Journal of Phytoremediation, 27(1), 128-134. https://doi.org/10.1080/15226514.2024.2403037

[93] Mondal, S., Bar, S., Roy, D., Hazra, S., Mal, J., Bilal, M., & Mal, C. (2022). Genetic engineering strategies and degradation of pollutants using genetically engineered microorganisms (GEMs). In Biotechnology for Environmental Protection (pp. 209-232). Singapore: Springer Nature Singapore. https://doi.org/10.1007/978-981-19-4937-1_8

[94] Goswami, D., Mukherjee, J., Mondal, C., & Bhunia, B. (2024). Bioremediation of azo dye: A review on strategies, toxicity assessment, mechanisms, bottlenecks and prospects. Science of The Total Environment, 954, 176426. https://doi.org/10.1016/j.scitotenv.2024.176426

[95] Alzain, H., Kalimugogo, V., Hussein, K., & Karkadan, M. (2023). A review of bacterial degradation of azo dyes. Int J Res Rev, 10, 443-462. https://doi.org/10.52403/ijrr.20230657

[96] Mushtaq, A., Cheema, A. I., Mahmood, F., Khan, M. A., Naqqash, T., Khurshid, M., ... & Shāhid, M. (2020). Heterologous expression of azoreductase-encoding gene azrS of Bacillus sp. MR-1/2 for enhanced azo dye decolorization and wastewater treatment. Archives of microbiology. https://doi.org/10.1007/s00203-020-01940-w

[97] Guo, H., Zheng, B., Jiang, D., & Qin, W. (2017). Overexpression of a laccase with dye decolorization activity from Bacillus sp. induced in Escherichia coli. Journal of Molecular Microbiology and Biotechnology, 27(4), 217-227. https://doi.org/10.1159/000478859

[98] Dixit, S., & Garg, S. (2019). Development of an efficient recombinant bacterium and its application in the degradation of environmentally hazardous azo dyes. International Journal of Environmental Science and Technology, 16(11), 7137-7146. https://doi.org/10.1007/s13762-018-2054-7

[99] Ayub, A., Wani, A. K., Chopra, C., Sharma, D. K., Amin, O., Wani, A. W., ... & Singh, R. (2025). Advancing dye degradation: integrating microbial metabolism, photocatalysis, and nanotechnology for eco-friendly solutions. Bacteria, 4(1), 15. https://doi.org/10.3390/bacteria4010015

[100] Sharma, M., Sharma, S., Alkhanjaf, A. A. M., Arora, N. K., Saxena, B., Umar, A., ... & Baskoutas, S. (2025). Microbial fuel cells for azo dye degradation: A perspective review. Journal of Industrial and Engineering Chemistry, 142, 45-67. https://doi.org/10.1016/j.jiec.2024.07.031

[101] Raj, A., Gauba, P., & Bhatt, E. (2025). Advanced nanoparticles for environmental remediation of emerging pollutants: a review. Soil and Sediment Contamination: An International Journal, 34(6), 1344-1373. https://doi.org/10.1080/15320383.2024.2422387

[102] Dutta, V., Verma, R., Gopalkrishnan, C., Yuan, M. H., Batoo, K. M., Jayavel, R., ... & Ghotekar, S. (2022). Bio-inspired synthesis of carbon-based nanomaterials and their potential environmental applications: a state-of-the-art review. Inorganics, 10(10), 169. https://doi.org/10.3390/inorganics10100169

[103] Ming, J., Ni, S. Q., Guo, Z., Wang, Z. B., & Xie, L. (2025). Photocatalytic material–microorganism hybrid systems in water decontamination. Trends in Biotechnology, 43(5), 1031-1047.

https://doi.org/10.1016/j.tibtech.2024.11.012

[104] Majul, L., Wirth, S., & Levin, L. (2022). High dye removal capacity of Peniophora laxitexta immobilized in a combined support based on polyurethane foam and lignocellulosic substrates. Environmental Technology, 43(5), 684-695. https://doi.org/10.1080/09593330.2020.1801851

[105] Modi, S., Yadav, V. K., Amari, A., Osman, H., Igwegbe, C. A., & Fulekar, M. H. (2023). Nanobioremediation: a bacterial consortium-zinc oxide nanoparticle-based approach for the removal of methylene blue dye from wastewater. Environmental Science and Pollution Research, 30(28), 72641-72651. https://doi.org/10.1007/s11356-023-27507-y

[106] Khan, N., Ahmad, A., Sharma, V., Saha, A. K., Pandey, A., & Bhargava, P. C. (2022). An integrative study for efficient removal of hazardous azo dye using microbe-immobilized cow dung biochar in a continuous packed bed reactor. Renewable Energy, 200, 1589-1601. https://doi.org/10.1016/j.renene.2022.10.016

[107] Wang, L., Cheng, D., Liu, X., & Ye, Y. (2024). Utilizing microorganisms immobilized on carbon-based materials for environmental remediation: a mini review. Water Emerging Contaminants & Nanoplastics, 3(3), N-A. https://doi.org/10.20517/wecn.2024.14

[108] Wahab, W. A. A. (2025). Review of research progress in immobilization and chemical modification of microbial enzymes and their application. Microbial cell factories, 24(1), 167. https://doi.org/10.1186/s12934-025-02791-0

[109] Abdelhamid, M. A., Khalifa, H. O., Yoon, H. J., Ki, M. R., & Pack, S. P. (2024). Microbial immobilized enzyme biocatalysts for multipollutant mitigation: harnessing nature’s toolkit for environmental sustainability. International journal of molecular sciences, 25(16), 8616. https://doi.org/10.3390/ijms25168616

[110] Pratama, S. A., Purnomo, A. S., Ediati, R., Asranudin, A., & Kusumawardhani, N. A. (2025). Novel biocomposite of Pseudomonas aeruginosa supported by metal-organic framework UiO-66 in sodium alginate-polyvinyl alcohol matrices for methylene blue decolorization: Effect of crosslinking agents and optimization using response surface methodology. International Journal of Biological Macromolecules, 305, 141016. https://doi.org/10.1016/j.ijbiomac.2025.141016

[111] Wang, T., Yang, M., Liang, J., Hu, L., Yang, J., Liu, H., ... & Liang, H. (2025). Magnetic biochar-facilitated azo dye degradation in bioelectrochemical system: Mechanistic insights from carbon metabolism, electron transfer, and molecular docking. Journal of Hazardous Materials, 140017. https://doi.org/10.2139/ssrn.5369608

[112] Purnomo, A. S., Prasetyoko, D., El Messaoudi, N., Rohmah, A. A., Hidayat, A. R. P., & Subagyo, R. (2024). Adsorption and biodegradation of the azo dye methyl orange using Ralstonia pickettii immobilized in polyvinyl alcohol (PVA)–alginate–hectorite beads (BHec-RP). RSC advances, 14(26), 18277-18290. https://doi.org/10.1039/d3ra08692e

[113] Budlayan, M. L. M., Patricio, J. N., Palangyos, D. C., Guerrero, R. A., & Arco, S. D. (2025). Direct Integration of Iron Oxide Nanoparticles on Bacterial Cellulose for Dye Degradation in Water. Solid State Phenomena, 369, 67-73. https://doi.org/10.4028/p-uwk5pj

[114] Chen, R. P., Cai, J. L., Li, Q., Yu, L., Wei, X. Y., Gan, C. H., ... & Yong, Q. (2021). Enhancement on the microbial extracellular electron transfers by modified lignin materials: application on decolorization of azo dye. Journal of Materials Research and Technology, 15, 5265-5276. https://doi.org/10.21203/rs.3.rs-419698/v1

[115] Pandey, S., Awasthee, N., Shekher, A., Yadav, P., Rai, L. C., Gupta, S. C., & Dubey, S. K. (2024). Microcystis aeruginosa mediated biofabrication of silver nanoparticles exhibiting antibacterial, antioxidant, anticancer, and azo dye degrading catalytic activities. Biomass Conversion and Biorefinery, 14(20), 25513-25531. https://doi.org/10.1007/s13399-023-04670-3

[116] Trivedi, P., Kumar, A., Gupta, N., & Patel, C. N. (2025). Artificial intelligence and machine learning in microbial degradation of pollutants and toxins. In Microbial Metabolomics: Recent Developments, Challenges and Future Opportunities (pp. 377-400). Singapore: Springer Nature Singapore. https://doi.org/10.1007/978-981-96-4824-5_16

[117] Patil, G. B., Nangare, S. N., Patil, S. M., Rajput, S. S., & Patil, M. M. (2025). AI-based models for prediction of biodegradation. In Artificial intelligence for chemical sciences (pp. 247-290). Apple Academic Press. https://doi.org/10.1201/9781003569282-12

[118] Bibi, M., Yasmin, A., Murtza, I., Saeed, A., Jamil, M. A., Syed, S. S., ... & Hai, A. (2025). Degradation of Anthraquinone dye Remazol Brilliant Blue R through Geobacillus laccase: Simulation of molecular docking and process optimization by artificial neural network. Results in Engineering, 106908. https://doi.org/10.1016/j.rineng.2025.106908

[119] Ahmad, Z., Zhong, H., Mosavi, A., Sadiq, M., Saleem, H., Khalid, A., ... & Nabipour, N. (2020). Machine learning modeling of aerobic biodegradation for azo dyes and hexavalent chromium. Mathematics, 8(6), 913. https://doi.org/10.3390/math8060913

[120] Pal, S., Jayaseelan, A., Rathankumar, A. K., Kumar, P., & Vishnu, D. (2026). Zirconium Nanomaterials for Treatment of Wastewater: Augmenting Antimicrobial Effectiveness and Optimization Through Artificial Intelligence Integration. International Journal of Environmental Research, 20(1), 25. https://doi.org/10.1007/s41742-025-00988-0

[121] Anandhi, G., & Iyapparaja, M. (2024). Photocatalytic degradation of drugs and dyes using a maching learning approach. RSC advances, 14(13), 9003-9019. https://doi.org/10.1039/d4ra00711e

[122] Srivastava, Y., & Jaiswal, R. P. (2025). Bioremediation of a dis-azo RB222 dye using a novel bacillus strain: Experimental and artificial neural network based process optimization. Water, Air, & Soil Pollution, 236(1), 21. https://doi.org/10.1007/s11270-024-07668-3

[123] Meena, M., Yadav, G., Sonigra, P., & Shah, M. P. (2022). A comprehensive review on application of bioreactor for industrial wastewater treatment. Letters in Applied Microbiology, 74(2), 131-158. https://doi.org/10.1111/lam.13557

[124] Saravanan, A., Ragini, Y. P., Karishma, S., Kamalesh, R., & Vickram, A. S. (2024). Review on the mechanisms and emerging prospects of biocatalytic dye degradation: Reactor systems and optimization strategies. Groundwater for Sustainable Development, 27, 101376. https://doi.org/10.1016/j.gsd.2024.101376

[125] Tekere, M. (2019). Microbial bioremediation and different bioreactors designs applied. In Biotechnology and bioengineering. IntechOpen. https://doi.org/10.5772/intechopen.83661

[126] Khan, M. D., Abdulateif, H., Ismail, I. M., Sabir, S., & Khan, M. Z. (2015). Bioelectricity generation and bioremediation of an azo-dye in a microbial fuel cell coupled activated sludge process. PLoS One, 10(10), e0138448. https://doi.org/10.1371/journal.pone.0138448

[127] Zabłocka-Godlewska, E., & Przystaś, W. (2020). Fed-batch decolourization of mixture of Brilliant Green and Evans Blue by bacteria species applied as pure and mixed cultures: influence of growth conditions. Water, Air, & Soil Pollution, 231(2), 75. https://doi.org/10.1007/s11270-020-4441-1

[128] Rai, V. K., Yadav, S., Saifi, G., Tiwari, H., & Singh, R. S. (2025). A comprehensive study of biodegradation and detoxification of triazine-based azo dye (Reactive Red 120) using biofilm-mediated continuous packed-bed bioreactor. Environmental Technology, 1-16. https://doi.org/10.1080/09593330.2025.2592739

[129] Owais, Muhammad, Asad A. Zaidi, Abdul Hameed Memon, Ahmad Hussain, and Arsalan Ahmed. "Assay of p-chlorophenol compliance monitoring in textile wet processing industry effluent using Fenton oxidation process." Journal of Ecological Engineering 24, no. 11 (2023). https://doi.org/10.12911/22998993/171370

[130] Shalini, & Setty, Y. P. (2019). Multistage fluidized bed bioreactor for dye decolorization using immobilized polyurethane foam: a novel approach. https://doi.org/10.1016/j.bej.2019.107368

[131] Samykannu, M. (2025). Eco-friendly approaches to azo dye removal: the role of microbial azo-reductases. Applied Biochemistry and Biotechnology, 1-19. https://doi.org/10.1007/s12010-025-05343-9

[132] Lach, C. E., Silveira, D. D., Belli, T. J., Lapolli, F. R., & Lobo-Recio, M. Á. (2026). Bioelectrochemical hybrid system integrating anodic biofilm and cathodic Fenton into a microbial fuel cell for multifunctional treatment of azo dye wastewater. Bioresource Technology, 446, 134149. https://doi.org/10.2139/ssrn.5947685

[133] Farastoon Dashti, S. S., Ansari, I., Emamshoushtari, M. M., Helchi, S., Lessage, G., Heran, M., & Pajoum Shariati, F. (2025). Comparison of batch and continuous operation modes for maxilon red azo dye removal using Chlorella vulgaris microalgae within photobioreactor (PBR) and a dynamic membrane photobioreactor (DMPBR). Frontiers in Membrane Science and Technology, 4, 1653159. https://doi.org/10.3389/frmst.2025.1653159

[134] Amacosta, J., Poznyak, T., Siles, S., & Chairez, I. (2024). Sequential treatment by ozonation and biodegradation of pulp and paper industry wastewater to eliminate organic contaminants. Toxics, 12(2), 138.

https://doi.org/10.5772/56011

[135] Castillo-Suárez, L. A., Sierra-Sánchez, A. G., Linares-Hernández, I., Martínez-Miranda, V., & Teutli-Sequeira, E. A. (2023). A critical review of textile industry wastewater: green technologies for the removal of indigo dyes. International Journal of Environmental Science and Technology, 20(9), 10553-10590. https://doi.org/10.1007/s13762-023-04810-2

[136] Woo, S., Jung, H., & Yoon, Y. (2023). Real-time UV/VIS spectroscopy to observe photocatalytic degradation. Catalysts, 13(4), 683. https://doi.org/10.3390/catal13040683

[137] Moharrery, L., Ardestani, N. S., & Otadi, M. (2025). Bio decolorization of the oil soluble azo dye toluidine Red by Halomonas strain A3. Scientific Reports, 15(1), 33249. https://doi.org/10.1038/s41598-025-18658-8

[138] Lohrasbi, M., Pattanapanishsawat, P., Isenberg, M., & Chuang, S. S. (2013, May). Degradation study of dye-sensitized solar cells by electrochemical impedance and FTIR spectroscopy. In 2013 IEEE Energytech (pp. 1-4). IEEE. https://doi.org/10.1109/energytech.2013.6645304

[139] Ahmadi, B., Fallah, A., Ghamarpoor, R., & Jamshidi, M. (2025). Methylene blue beyond the dye: A critical review on its role as a benchmark pollutant in photocatalyst design. Results in Chemistry, 102910. https://doi.org/10.1016/j.rechem.2025.102910

[140] Gayathri, K., Saranraj, P., Nayak, A. K., Kesavardhini, K., Lokeshwari, B., & Cardoso, A. M. (2026). Microbial Innovations for Sustainable Wastewater Management: A Comprehensive Review of Azo Dye Bioremediation. Sustainability, 18(6), 3041. https://doi.org/10.3390/su18063041

[141] Wong, J. K. H., Tan, H. K., Lau, S. Y., Yap, P. S., & Danquah, M. K. (2019). Potential and challenges of enzyme incorporated nanotechnology in dye wastewater treatment: A review. Journal of environmental chemical engineering, 7(4), 103261. https://doi.org/10.1016/j.jece.2019.103261

[142] Periyasamy, A. P. (2025). Textile dyes in wastewater and its impact on human and environment: focus on bioremediation. Water, Air, & Soil Pollution, 236(9), 562 https://doi.org/10.1007/s11270-025-08204-7

Downloads

Published

2026-04-23

Data Availability Statement

No new data were generated or analyzed in this study. All information presented is derived from previously published studies, which have been appropriately cited in the manuscript.

Issue

Section

Review Articles

How to Cite

[1]
H. Islam, “Role of Microbial Diversity in Textile Dye Degradation: A Comprehensive Review”, JENMAS, vol. 2, no. 1, pp. 55–78, Apr. 2026, doi: 10.66173/jenmas.2026.55.